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| Registros recuperados: 76 | |
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| Gagne, Renee; Tremblay, Rejean; Pernet, Fabrice; Miner, Philippe; Samain, Jean-francois; Olivier, Frederic. |
| The main objective of this study was to evaluate the effect of the addition of Rhodomonas salina in the diet of Pecten maximus on growth, survival and metamorphosis success in relation to biochemical content. Food quality is an essential factor in the success of larval, post-larval and juvenile development. A diet rich in polyunsaturated fatty acids present an advantage for growth, survival and metamorphosis success. Larvae and post-larvae fed with the diet containing R. sauna showed a higher accumulation of arachidonic acid (20:4n-6) and generally a lower concentration of docosahexaenoic acid (22:6n-3). Addition of R. sauna in diet was related with higher level of eicosapentaenoic acid (20:5n-3) in larval stages, but lower level in post-larvae. Addition... |
| Tipo: Text |
Palavras-chave: Nutrition; Biochemical characteristics; Fatty acids; Sterol profiles. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00019/13045/10205.pdf |
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| Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice. |
| Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary... |
| Tipo: Text |
Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf |
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| Rayssac, Nathalie; Malet, Nathalie; Pernet, Fabrice. |
| The objective of this work is to understand why oysters Crassostrea gigas shows low settlement success in the Thau lagoon despite the fact that biomass of adults is elevated and that larvae show normal development in the water column. Our hypotheses are that oysters compete for food or space with other filter-feeders animals such as sponges, ascidians and other bivalves. To test the trophic hypothesis, we analysed the entire settler community harvested on four-month-old collectors deployed in the Thau lagoon during the summer 2009 for stable isotopes and fatty acids. Based on the isotopic, crustacean amphipods and barnacles can be rejected as a potential food competitor of juvenile oysters. However, other bivalves such as Mytilus sp., Anomia sp., and... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12665/9555.pdf |
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| Pernet, Fabrice; Malet, Nathalie; Pastoureaud, Annie; Vaquer, Andre; Quere, Claudie; Dubroca, Laurent. |
| Carbon stable isotopes and fatty acids were measured in the suspended particulate organic matter (POM) of the Thau lagoon to study its qualitative temporal changes in relation to environmental factors and to identify the food sources of bivalves over a one-yr-cycle in relation to their growth. Reciprocally, the impact of shellfish farming on POM was also studied. Oysters and mussels were sampled and measured for biometry, stable isotopes and fatty acid composition. Water samples were collected at two sites, both inside and outside of the shellfish farming area, to determine concentrations in POM, chlorophyll a (Chl a) and stable isotopes. Carbon isotopes and fatty acids in bivalves reflected seasonal changes in food sources, which varied consistently with... |
| Tipo: Text |
Palavras-chave: Aquaculture; Bivalve; Competition; Feeding ecology; Growth; Trophic markers. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00075/18575/16177.pdf |
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| Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Corporeau, Charlotte; Degremont, Lionel; Lagarde, Franck; Pepin, Jean-francois; Keck, Nicolas. |
| Experiments were conducted to investigate the effects of farming sites, seeding date, age of oysters, ploidy level and rearing structures on mortality, pathogens and energetic reserves of oysters Crassostrea gigas. Mortality of oysters coincided with infections involving, primarily, the ostreid herpesvirus OsHV-1 and, secondarily, bacteria of the group Vibrio splendidus, when seawater temperature was between 17 and 24°C. Mortality started in early May and lasted until late September, with an interruption in July and August when seawater temperatures rose above 24°C. Mortality varied with farming site: oysters maintained in the open Mediterranean Sea showed no mortality and OsHV-1 was occasionally detected at a low level only, while oysters maintained in... |
| Tipo: Text |
Palavras-chave: Pacific oyster; Mortality; Disease; Farming practices; Hydrodynamics; Energetic. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00086/19739/17379.pdf |
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| Toupoint, Nicolas; Gilmore-solomon, Lisandre; Bourque, Francois; Myrand, Bruno; Pernet, Fabrice; Olivier, Frederic; Tremblay, Rejean. |
| We considered Cushing's match/mismatch theory in a heterotrophic environment and hypothesized that settlement and recruitment success in blue mussel are higher when the food supply is rich in polyunsaturated and essential fatty acids (PUFA/EFA). To test this hypothesis, we monitored larval development as well as fatty acid composition in trophic resources during two successive reproductive seasons. The decoupling we found between the presence of competent larvae in the water column and settlement rates strongly suggests that metamorphosis is delayed until conditions are suitable. In both years, the major mussel settlement peak was synchronized with a phytoplanktonic pulse rich in EFA, consisting of a large autotrophic bloom in 2007 and a short but... |
| Tipo: Text |
Palavras-chave: Fatty acids; Larval supply; Match/mismatch; Mytilus edulis; Picoeukaryotes; Recruitment; Settlement rate; Trophic settlement trigger. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00098/20923/19676.pdf |
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| Brosset, Pablo; Fromentin, Jean-marc; Menard, Frederic; Pernet, Fabrice; Bourdeix, Jean-herve; Bigot, Jean-louis; Van Beveren, Elisabeth; Perez Rodia, Maria A.; Choy, Sandrine; Saraux, Claire. |
| As condition is a key variable in population dynamics (especially for survival, growth and reproduction), the use of well-defined and accurate fish condition indices is capital. In particular, condition indices (morphometric, bioenergetic and biochemical) have never been compared and validated for the European anchovy Engraulis encrasicolus, the European pilchard Sardina pilchardus and the European sprat Sprattus sprattus. The accuracy of two indirect methods, the morphometric relative condition index Kn and the bioenergetics index determined with the Distell Fish Fatmeter was investigated by comparing with a direct measure of relative lipid content carried out with a thin layer chromatography-flame ionization detector. Estimations from the fatmeter... |
| Tipo: Text |
Palavras-chave: Anchovy; Fatmeter; Lipids; Mediterranean Sea; Sardine; Sprat. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00226/33741/32304.pdf |
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| Richard, Gaelle; Guerard, Fabienne; Corporeau, Charlotte; Lambert, Christophe; Paillard, Christine; Pernet, Fabrice. |
| We investigated the effect of brown ring disease (BRD)1 development and algal diet on energy reserves and activity of enzymes related to energy metabolism, antioxidant system and immunity in Manila clam, Ruditapes philippinarum. We found that algal diet did not impact the metabolic response of clams exposed to Vibrio tapetis. At two days post-injection (dpi), activities of superoxide dismutase and glutathione peroxidase (GPx) decreased whereas activities of nitric oxide synthase (iNOS) and catalase increased in infected clams, although no clinical signs were visible (BRD-). At 7 dpi, activities of several antioxidant and immune-related enzymes were markedly increased in BRD-likely indicating an efficient reactive oxygen species (ROS) scavenging compared to... |
| Tipo: Text |
Palavras-chave: Antioxidant enzymes; Immunity; Energetic metabolism; Ruditapes philippinarum; Vibrio tapetis. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00316/42684/42057.pdf |
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| Fuhrmann, Marine; Delisle, Lizenn; Petton, Bruno; Corporeau, Charlotte; Pernet, Fabrice. |
| The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and... |
| Tipo: Text |
Palavras-chave: Bivalve; Disease; Environment; Metabolism; Mortality risk; Salinity. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00427/53886/54958.pdf |
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| Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Malet, Nathalie; Pastoureaud, Annie; Munaron, Dominique; De Lorgeril, Julien; Bachere, Evelyne; Vaquer, Andre; Huvet, Arnaud; Corporeau, Charlotte; Normand, Julien; Boudry, Pierre; Moal, Jeanne; Quere, Claudie; Quilien, Virgile; Daniel, Jean-yves; Pepin, Jean-francois; Saulnier, Denis; Gonzalez, Jean-louis. |
| This study was conducted in the Mediterranean area during a mass mortality event of Pacific oyster Crassostrea gigas. The objectives of this work were to increase our understanding of the mass mortality phenomena in Pacific oysters, to identify risky cultivation practises and to propose mitigation strategies. In the first part of this work, we followed environmental parameters of the Thau lagoon in relation with mortality events. Then, we compared mortality and growth parameters of oysters as a function of their origins, their development stages and cultivation sites in relation with energetic, reproduction and pathogens. Finally, we conducted transfer experiments where oysters maintained free of mortality in open sea were transferred in the Thau lagoon... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11354/7927.pdf |
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| Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Lagarde, Franck; Fiandrino, Annie; Huvet, Arnaud; Corporeau, Charlotte; Boudry, Pierre; Quere, Claudie; Degremont, Lionel; Pepin, Jean-francois; Saulnier, Denis; Boulet, Hélène; Keck, Nicolas. |
| The objectives of this study are to increase our understanding of the mass mortality phenomena in Pacific oysters Crassostrea gigas in the Thau lagoon and to examine the effect of cultivation practises. Mortality events in 2009 and 2010 always coincide with simple or dual infections involving primarily the Herpes virus OsHV-I and secondarily the bacteria Vibrio splendidus, when seawater temperature is between 17°C and 24°C. In the Thau lagoon, mortality starts early May and last until fall with an interruption period when seawater temperature is higher than 24°C in July and August. Mortality of oysters varies as a function of zootechnical life-history, hydrodynamics and cultivation practises. First, mortality depends on whether or not oysters have been... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15404/12764.pdf |
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| Fleury, Elodie; D'Amico, Florence; Annezo, Jean-pierre; Barret, Jean; Blin, Jean Louis; Bouget, Jean-francois; Claude, Serge; Guilpain, Patrice; Grizon, James; Hitier, Benoist; Langlade, Aime; Le Gall, Patrik; Le Souchu, Pierrick; Martin, Anne-genevieve; Mary, Charlotte; Normand, Julien; Parrad, Sophie; Penot, Julia; Pernet, Fabrice; Pien, Sebastien. |
| Depuis 2009, l'Observatoire Conchylicole assure le suivi, sur des sites ateliers disposés sur l'ensemble du littoral français, de lots sentinelles d'huîtres creuses Crassostrea gigas représentatifs de la production française. Leur suivi permet d'acquérir des données nationales de croissance et de mortalité, de traduire la dynamique spatio-temporelle des performances d’élevage et ainsi de participer à la compréhension des phénomènes observés. Pour ce faire, des lots sentinelles d’huîtres correspondant à différentes origines (captage ou écloserie, diploïdes ou triploïdes) et à différents stades d’élevage (naissain ou adultes 18 mois) sont déployés simultanément sur 13 sites ateliers représentant les grandes régions conchylicoles du littoral français. En... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00083/19433/17047.pdf |
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| Genard, Bertrand; Pernet, Fabrice; Lemarchand, Karine; Boudry, Pierre; Moraga, Dario; Tremblay, Rejean. |
| In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of... |
| Tipo: Text |
Palavras-chave: Massive mortality; Larvae; Microbial environment; Lipid; Fatty acids; Energy metabolism; Oxidative stress; Antioxidant enzymes; Crassostrea virginica. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16231/13753.pdf |
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| Tremblay, Rejean; Landry, Thomas; Leblanc, Neil; Pernet, Fabrice; Barkhouse, Carla; Sevigny, Jean-marie. |
| The bivalve's aquaculture industry is an important component of the economy in Eastern Canada. Seed collection is an initial and critical activity in most bivalve aquaculture industries including mussel farming in Prince Edward Island, production is entirely dependent on natural spat collection. Although seed supply is not a concern from a quantitative standpoint, there are growing concerns about the quality of natural seed. The general objective of this study was to identify and assess mussel seed quality criteria on the basis of physiological and biochemical status under laboratory and field conditions. The performance, as estimated by metabolic measurements, lipid class composition, multi-locus heterozygosity (MLH) and survival to stressful environment... |
| Tipo: Text |
Palavras-chave: Wild seed; Aquaculture; Physiological fitness; Growth; Survival; Multi-locus heterozygosity; Mytilus spp.. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16232/13754.pdf |
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| Rayssac, Nathalie; Pérignon, Adeline; Gervasoni, Erika; Pernet, Fabrice; Legall, Patrick; Lagarde, Franck. |
| INTRODUCTION - PRONAMED 2010-2011- Ce projet, initié le Comité Régional de Conchyliculture de Méditerranée (CRCM) et l’Ifremer a été mis en œuvre grâce à la collaboration du CRCM, du Cépralmar ainsi que du réseau VELYGER et du laboratoire environnement ressources du Languedoc Roussillon (LER-LR) de l’Ifremer. En outre, ce projet local a bénéficié de fonds d’état dans le cadre du programme de soutien à l’innovation dans la filière pêche et aquaculture proposé par FranceAgrimer, de fonds européens via le Fond d’Étude pour les Pêches, et de fonds publics locaux grâce aux subventions de la région Languedoc Roussillon et du conseil général de l’Hérault. PRÉAMBULE - La version initiale du projet PRONAMED comportait deux volets de recherche. Le premier, consacré... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00088/19959/17626.pdf |
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| Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie. |
| Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters... |
| Tipo: Text |
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf |
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| Petton, Bruno; Riobe, Marion; Koechlin, Hugo; Queau, Isabelle; Mingant, Christian; Ratiskol, Dominique; Le Grand, Jacqueline; Petton, Sebastien; Thomas, Yoann; Alunno-bruscia, Marianne; Pouvreau, Stephane; Pernet, Fabrice; Maurer, Daniele; D'Amico, Florence; Lagarde, Franck; Mortreux, Serge; Roque D'Orbcastel, Emmanuelle; Mille, Dominique; Geay, A.; Bouquet, A.-l.; Cochet, Helene. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Des travaux de qualification zoo-sanitaire menés depuis 2010 ont montré qu’un lot de naissains issus du captage naturel sur deux était infecté par le virus bien avant l’apparition des mortalités dans le milieu naturel, mais qu’a contrario les naissains d’écloserie expertisés présentaient un moindre risque de contamination. Dans le contexte de crise sanitaire chronique, l’objectif de notre étude était d’ obtenir une information qualitative relative au statut zoo-sanitaire OsHV -1 μ V ar des... |
| Tipo: Text |
Palavras-chave: Huitre creuse; OsHV - 1μVar; Epreuve thermique.; Maladie; Naissain; Pacific oyster; Spat; Disease; Thermal challenge; OsHV-1 μVar. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00276/38682/37180.pdf |
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| De Decker, Sophie; Normand, Julien; Saulnier, Denis; Pernet, Fabrice; Castagnet, Sophie; Boudry, Pierre. |
| Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio... |
| Tipo: Text |
Palavras-chave: Experimental vibriosis; Crassostrea gigas; Vibrio; Survival distribution function; Triploidy; Reproductive effort. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00030/14108/11368.pdf |
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| Registros recuperados: 76 | |
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